Cloning and Expression of the Nucleocapsid Gene of Virulent Kabete O Strain of Rinderpest Virus in Baculovirus: Use in Differential Diagnosis between Vaccinated and Infected Animals
Identifieur interne : 004471 ( Main/Exploration ); précédent : 004470; suivant : 004472Cloning and Expression of the Nucleocapsid Gene of Virulent Kabete O Strain of Rinderpest Virus in Baculovirus: Use in Differential Diagnosis between Vaccinated and Infected Animals
Auteurs : Tarek Ismall [États-Unis] ; Shabbir Ahmad [États-Unis] ; Marian D'Souza-Ault [États-Unis] ; Mansour Bassiri [États-Unis] ; Jeremiah Saliki [États-Unis] ; Charles Mebus [États-Unis] ; Tilahun Yilma [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: Rinderpest (RP) is a viral disease of ruminants with >95% morbidity and mortality. We have cloned the cDNA of the nucleocapsid (N) gone of the virulent Kabete O strain of rinderpest virus (RPVK) and compared its nucleotide and deduced amino acid sequences with those of the N genes of the lapinized strain of rinderpest virus (RPVL), measles virus (MV), and canine distemper virus (CDV). The gene has an open reading frame of 1575 nucleotides and encodes a protein of 525 amino acids with a molecular weight of 59 kDa. The nucleotide sequence of the coding region of the N gone of RPVK is 88.6, 68.9, and 63.2% homologous with N genes of RPVL, MV, and CDV, respectively. We have developed a recombinant baculovirus that expresses the N protein (rRVN) of RPVK in insect cells (Sf9) and larvae (Spodoptera frugiperda). rRVN was used as a coating antigen in an ELISA to distinguish vaccinated animals from those infected with RPV and was also used successfully in the diagnosis of two other morbilliviruses, MV and peste des petits ruminants (PPRV). Crude lysate of a single infected larva (0.2-0.3 g) was sufficient to coat 150 ELISA plates for serological diagnosis of 7200 serum samples in duplicate.
Url:
DOI: 10.1006/viro.1994.1016
Affiliations:
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Box 848, Greenport</wicri:cityArea></affiliation></author><author><name sortKey="Ahmad, Shabbir" sort="Ahmad, Shabbir" uniqKey="Ahmad S" first="Shabbir" last="Ahmad">Shabbir Ahmad</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>International Laboratory of Molecular Biology for Tropical Disease Agents, Department of Veterinary Microbiology and Immunology, University of California, Davis, California 95616; and Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratory, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, P.O. Box 848, Greenport</wicri:cityArea></affiliation></author><author><name sortKey="D Souza Ault, Marian" sort="D Souza Ault, Marian" uniqKey="D Souza Ault M" first="Marian" last="D'Souza-Ault">Marian D'Souza-Ault</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>International Laboratory of Molecular Biology for Tropical Disease Agents, Department of Veterinary Microbiology and Immunology, University of California, Davis, California 95616; and Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratory, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, P.O. Box 848, Greenport</wicri:cityArea></affiliation></author><author><name sortKey="Bassiri, Mansour" sort="Bassiri, Mansour" uniqKey="Bassiri M" first="Mansour" last="Bassiri">Mansour Bassiri</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>International Laboratory of Molecular Biology for Tropical Disease Agents, Department of Veterinary Microbiology and Immunology, University of California, Davis, California 95616; and Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratory, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, P.O. Box 848, Greenport</wicri:cityArea></affiliation></author><author><name sortKey="Saliki, Jeremiah" sort="Saliki, Jeremiah" uniqKey="Saliki J" first="Jeremiah" last="Saliki">Jeremiah Saliki</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>International Laboratory of Molecular Biology for Tropical Disease Agents, Department of Veterinary Microbiology and Immunology, University of California, Davis, California 95616; and Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratory, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, P.O. Box 848, Greenport</wicri:cityArea></affiliation></author><author><name sortKey="Mebus, Charles" sort="Mebus, Charles" uniqKey="Mebus C" first="Charles" last="Mebus">Charles Mebus</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>International Laboratory of Molecular Biology for Tropical Disease Agents, Department of Veterinary Microbiology and Immunology, University of California, Davis, California 95616; and Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratory, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, P.O. Box 848, Greenport</wicri:cityArea></affiliation></author><author><name sortKey="Yilma, Tilahun" sort="Yilma, Tilahun" uniqKey="Yilma T" first="Tilahun" last="Yilma">Tilahun Yilma</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>International Laboratory of Molecular Biology for Tropical Disease Agents, Department of Veterinary Microbiology and Immunology, University of California, Davis, California 95616; and Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratory, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, P.O. Box 848, Greenport</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">198</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="138">138</biblScope><biblScope unit="page" to="147">147</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Rinderpest (RP) is a viral disease of ruminants with >95% morbidity and mortality. We have cloned the cDNA of the nucleocapsid (N) gone of the virulent Kabete O strain of rinderpest virus (RPVK) and compared its nucleotide and deduced amino acid sequences with those of the N genes of the lapinized strain of rinderpest virus (RPVL), measles virus (MV), and canine distemper virus (CDV). The gene has an open reading frame of 1575 nucleotides and encodes a protein of 525 amino acids with a molecular weight of 59 kDa. The nucleotide sequence of the coding region of the N gone of RPVK is 88.6, 68.9, and 63.2% homologous with N genes of RPVL, MV, and CDV, respectively. We have developed a recombinant baculovirus that expresses the N protein (rRVN) of RPVK in insect cells (Sf9) and larvae (Spodoptera frugiperda). rRVN was used as a coating antigen in an ELISA to distinguish vaccinated animals from those infected with RPV and was also used successfully in the diagnosis of two other morbilliviruses, MV and peste des petits ruminants (PPRV). Crude lysate of a single infected larva (0.2-0.3 g) was sufficient to coat 150 ELISA plates for serological diagnosis of 7200 serum samples in duplicate.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Ismall, Tarek" sort="Ismall, Tarek" uniqKey="Ismall T" first="Tarek" last="Ismall">Tarek Ismall</name></region><name sortKey="Ahmad, Shabbir" sort="Ahmad, Shabbir" uniqKey="Ahmad S" first="Shabbir" last="Ahmad">Shabbir Ahmad</name><name sortKey="Bassiri, Mansour" sort="Bassiri, Mansour" uniqKey="Bassiri M" first="Mansour" last="Bassiri">Mansour Bassiri</name><name sortKey="D Souza Ault, Marian" sort="D Souza Ault, Marian" uniqKey="D Souza Ault M" first="Marian" last="D'Souza-Ault">Marian D'Souza-Ault</name><name sortKey="Mebus, Charles" sort="Mebus, Charles" uniqKey="Mebus C" first="Charles" last="Mebus">Charles Mebus</name><name sortKey="Saliki, Jeremiah" sort="Saliki, Jeremiah" uniqKey="Saliki J" first="Jeremiah" last="Saliki">Jeremiah Saliki</name><name sortKey="Yilma, Tilahun" sort="Yilma, Tilahun" uniqKey="Yilma T" first="Tilahun" last="Yilma">Tilahun Yilma</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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